草坪阔叶杂草防除技术研究

在2001～2002年两年间,应用百草敌、2,4-D丁酯乳油、2-甲-4-氯丙酸及混合药剂对青海大学农牧学院草业科学系试验地草坪杂草进行防除试验。结果表明:百草敌、2,4-D丁酯乳油及混合药剂I对几种主要杂草防除效果比较好,与对照相比30 d能达100％,而且两种混合药剂有增效作用,扩大了除草剂的使用范围。     

Comparative phosphoproteome analysis of cardiomyocytes preconditioned by diazoxide

AIM: To analyze and identify the phosphoproteins associated with diazoxide preconditioning. METHODS: Proteomics technique was used to investigate the changes of phosphoprotein after diazoxide preconditioning. Adult rat ventricular myocytes were pretreated in the presence and absence of 200 μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched respectively from control and diazoxide pretreated groups were then separated by two-dimensional (2D) gel electrophoresis and stained with sliver staining kit. Phosphoproteins of interest were further identified by mass spectrometry. RESULTS: Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP_346548 were phosphorylated significantly. The proteins of 94 kD glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly (P<0.05). CONCLUSION: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in response to diazoxide preconditioned signaling, which may mediate myocardioprotection signaling downstream mitochondrial staining dish K_ATP channel induced by ischemic preconditioning.     

Increased expression and possible roles of nicotinamide N-methyltransferase in pancreatic islets of STZ-induced diabetic monkeys

AIM: To verify and localize the expression of nicotinamide N-methyltransferase (NNMT) in pancreas of streptozotocin(STZ)-induced diabetic monkeys and understand its important role in β-cell destruction in the pathogenesis of diabetes. METHODS: Through an olig-microarray gene chip, NNMT was identified as the most obviously up-regulated genes in pancreas of STZ-induced diabetic monkeys versus controls. Semiquantitative RT-PCR and Western blotting were performed to verify the differential expression at mRNA and protein level respectively. Then the cellular localization of NNMT expression within pancreas was identified by immunohistochemical(IHC) staining.RESULTS: An obvious high expression of NNMT at both mRNA and protein levels was shown in pancreas of STZ-induced diabetic monkeys compared to that of controls. Further localization of the protein by IHC staining in pancreas specimens showed that its altered expression was restricted to central islets, most of which were β cells.CONCLUSION: Expression of NNMT is increased in islets of STZ- induced diabetic monkeys, which infers that NNMT might participate in the process of β cell damage in diabetes probably through the mechanism of energy metabolism disturbance.     

克雷伯氏菌胞外多糖的制备与组成分析

利用不同血清型的克雷伯氏菌Klebsiella菌株液体发酵,制备胞外多糖,并采用气相色谱和化学分析方法分析胞外多糖的化学组成。结果表明,菌株K26产胞外多糖的能力最高,培养48h和72h的产量分别为3.11mg/ml和3.89mg/ml;各实验菌株的胞外多糖的单糖组成包括甘露糖、葡萄糖和半乳糖。     

双向电泳结合蛋白质印迹技术分析猪囊尾蚴乳酸脱氢酶

为了研究猪囊尾蚴乳酸脱氢酶(LDH)的表达,试验选择四川省雅江县呷拉乡猪带绦虫病患者,给其口服槟榔-南瓜子驱虫,收集、制备虫卵悬液(8万个/mL),再给3头20日龄三元杂交乳猪猪灌胃虫卵悬液,每头1 mL,40 d后收集、制备猪囊尾蚴蛋白,进行双向电泳(2-DE)分析,将凝胶蛋白斑点转移至聚偏氟乙烯膜(PVDF膜),用自制的大鼠抗猪带绦虫LDH血清作为一抗、健康大鼠血清作为阴性对照进行蛋白质印迹(Western-blotting)分析。结果表明:双向电泳凝胶共检测到(207±9)个蛋白质斑点,相对分子质量(Mr)为14 400～94 000,等电点(pI)为3.0～10.0;试验组特异性抗原抗体阳性杂交斑点为1个,阴性对照未见阳性杂交斑点;将Western-blotting检测的抗原抗体阳性杂交斑点与原双向电泳凝胶斑点进行比对,找到对应蛋白斑点,经ImageMaster 2D Platinum 5.0软件分析后初步确定该蛋白斑点的pI/Mr为7.03/35 368,与猪带绦虫LDH的pI/Mr理论推导值接近,说明猪囊尾蚴表达LDH。     

中国金鱼反季节繁殖培育技术的初步研究

在低温季节,采用人工控温、营养控制和注射外源激素的方法,对金鱼(鹤顶红和红狮头)进行催产试验和鱼苗培育试验.结果表明,在(21±1)℃条件下,雌雄金鱼性腺成熟,并顺利产卵,受精率在90%以上,孵化率最高达到85%,仔稚鱼成活率为80%以上,完全达到自然繁殖时相应的生产指标.同时对胚胎发育过程也进行了观察,受精卵能正常发育,孵化时间为79 h 22 min.     

木本能源植物文冠果的表型多样性研究

以文冠果为研究对象,调查了14个分布区文冠果的15个表型性状,利用变异系数和巢式方差分析研究了分布区间和分布区内的变异情况;应用相关分析揭示了表型性状间及其与地理因子间的关系;运用聚类分析进行了分布区分类.结果表明,文冠果分布区内的变异大于分布区间的变异,分布区间的分化相对较小;出种数随经度的增大而减少,果宽随纬度的增大而增大,但随年平均气温的增大而减小,而其它13个性状随地理位置的改变没有特别明显的变化;表型性状的欧式距离与地理距离相关不显著.     

海岸基干林带作业设计新理念探索——以浙江瑞安市海岸基干林带建设为例

以浙江瑞安市海岸基干林带建设为例,在基干林带内部结构方面,提出海岸基干林带为生态系统聚集体的新理念,把生态系统作为构成海岸基干林带的基本单元。瑞安市海岸基干林带通过扩充基干林带规模,增加水面空间,形成林水交融的多系统综合体,显著提高基干林带的防风滞浪滞洪功能;在基干林带功能定位方面,提出在强化基干林带防风护岸基本功能的前提下,加强基干林带滞洪泄洪和景观美化功能,建设具有复合功能的新型基干林带。瑞安市海岸基干林带充分利用林水交融的景观特点,加强观花、观叶、观果树种的营造,实现休闲娱乐空间在防护林带中的合理布局,不仅赋予基干林带生态防护功能,而且使其具有独特的生态旅游功能,与基干林带周边发达的社会经济条件相衔接,提高基干林带的自我经营能力。     

论现代居住区景观设计

本文对当前我国居住区景观建设现状进行了分析,总结了居民生活的基本规律,论述了居住区景观设计的总体原则、整体特色、个性特色、道路绿地设计和建筑小品设计。     

叶用莴苣叶片对LED脉冲光的光合响应模型及光能利用

为探究LED脉冲光对作物光合特性的影响,探讨在不影响作物正常生长的前提下如何进一步降低能耗,提高照光效率,选用叶用莴苣(Lactuca sative L.)为试验材料,设置5个占空比水平(20%、40%、60%、80%和100%)和14个频率水平(1、2、4、8、16、32、64、128,256、512、1 024、2 048、4 096和8 192 Hz),共计70个处理,以连续光(占空比100%)为对照,探讨不同形式脉冲光对叶片净光合速率(Pn)的影响,并构建脉冲光占空比和频率与净光合速率之间的响应模型。通过对模型边际效应、交互作用以及光能利用率(LUE)的分析得出:占空比和频率都对 Pn 增长有促进作用,随着占空比和频率的增加,Pn也随之增加并最终趋于稳定状态直至与连续光处理的Pn无显著差异;占空比越高,叶用莴苣叶片的Pn达到饱和的频率越低;当Pn达到饱和状态时,脉冲光下的LUE要显著高于连续光。综上,占空比20%、频率512 Hz为最佳占空比与脉冲光频率组合。该结果可为优化LED植物节能补光灯光源参数设计及研发提供理论支撑。